5ok8

Crystal structure of protein Lpp20 (HP1456) from Helicobacter pylori

spinqualitylabelsall models 5ok8, resolution 1.87Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5ok8 is a 4 chain structure with sequence from Helicobacter pylori J99. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.874Å Ligands: CA, EPE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function LPP20_HELPJ Could play a role in the pathogenesis of H.pylori by serving as an inflammatory mediator. Publication Abstract from PubMed BACKGROUND: Helicobacter pylori is a bacterium that affects about 50% of the world population and, despite being often asymptomatic, it is responsible of several gastric diseases, from gastritis to gastric cancer. The protein Lpp20 (HP1456) plays an important role in bacterium survival and host colonization, but the possibility that it might be involved in the etiology of H. pylori-related disorders is an unexplored issue. Lpp20 is a lipoprotein bound to the external membrane of the bacterium, but it is also secreted inside vesicles along with other two proteins of the same operon, i.e. HP1454 and HP1457. RESULTS: In this study we determined the crystal structure of Lpp20 and we found that it has a fold similar to a carcinogenic factor released by H. pylori, namely Tipalpha. We demonstrate that Lpp20 promotes cell migration and E-cadherin down-regulation in gastric cancer cells, two events recalling the epithelial-mesenchymal transition (EMT) process. Differently from Tipalpha, Lpp20 also stimulates cell proliferation. CONCLUSIONS: This identifies Lpp20 as a new pathogenic factor produced by H. pylori that promotes EMT and thereby the progression of cancer to the metastatic state. Helicobacter pylori antigenic Lpp20 is a structural homologue of Tipalpha and promotes epithelial-mesenchymal transition.,Vallese F, Mishra NM, Pagliari M, Berto P, Codolo G, de Bernard M, Zanotti G Biochim Biophys Acta. 2017 Dec;1861(12):3263-3271. doi:, 10.1016/j.bbagen.2017.09.017. Epub 2017 Sep 22. PMID:28947343 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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