5t5k

Structure of histone-based chromatin in Archaea

spinqualitylabelsall models 5t5k, resolution 4.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5t5k is a 8 chain structure with sequence from Methanothermus fervidus and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 4Å Ligands: CAC Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function HMFB_METFE Binds and compact DNA (95 to 150 base pairs) to form nucleosome-like structures that contain positive DNA supercoils. Increases the resistance of DNA to thermal denaturation. Publication Abstract from PubMed Small basic proteins present in most Archaea share a common ancestor with the eukaryotic core histones. We report the crystal structure of an archaeal histone-DNA complex. DNA wraps around an extended polymer, formed by archaeal histone homodimers, in a quasi-continuous superhelix with the same geometry as DNA in the eukaryotic nucleosome. Substitutions of a conserved glycine at the interface of adjacent protein layers destabilize archaeal chromatin, reduce growth rate, and impair transcription regulation, confirming the biological importance of the polymeric structure. Our data establish that the histone-based mechanism of DNA compaction predates the nucleosome, illuminating the origin of the nucleosome. Structure of histone-based chromatin in Archaea.,Mattiroli F, Bhattacharyya S, Dyer PN, White AE, Sandman K, Burkhart BW, Byrne KR, Lee T, Ahn NG, Santangelo TJ, Reeve JN, Luger K Science. 2017 Aug 11;357(6351):609-612. doi: 10.1126/science.aaj1849. PMID:28798133 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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