5t9e

Seleno-methionine Prephenate Dehydrogenase from Soybean

spinqualitylabelsall models 5t9e, resolution 2.03Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5t9e is a 2 chain structure with sequence from Glycine max. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.03Å Ligands: MSE, NAP Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function I1MYY4_SOYBN Publication Abstract from PubMed L-Tyrosine (Tyr) is essential for protein synthesis and is a precursor of numerous specialized metabolites crucial for plant and human health. Tyr can be synthesized via two alternative routes by different key regulatory TyrA family enzymes, prephenate dehydrogenase (PDH, also known as TyrAp) or arogenate dehydrogenase (ADH, also known as TyrAa), representing a unique divergence of primary metabolic pathways. The molecular foundation underlying the evolution of these alternative Tyr pathways is currently unknown. Here we characterized recently diverged plant PDH and ADH enzymes, obtained the X-ray crystal structure of soybean PDH, and identified a single amino acid residue that defines TyrA substrate specificity and regulation. Structures of mutated PDHs co-crystallized with Tyr indicate that substitutions of Asn222 confer ADH activity and Tyr sensitivity. Reciprocal mutagenesis of the corresponding residue in divergent plant ADHs further introduced PDH activity and relaxed Tyr sensitivity, highlighting the critical role of this residue in TyrA substrate specificity that underlies the evolution of alternative Tyr biosynthetic pathways in plants. Molecular basis of the evolution of alternative tyrosine biosynthetic routes in plants.,Schenck CA, Holland CK, Schneider MR, Men Y, Lee SG, Jez JM, Maeda HA Nat Chem Biol. 2017 Jun 26. doi: 10.1038/nchembio.2414. PMID:28671678 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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