5u3e

Crystal Structure of Native Lectin from Canavalia bonariensis Seeds (CaBo) complexed with alpha-methyl-D-mannoside

spinqualitylabelsall models 5u3e, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5u3e is a 1 chain structure with sequence from Canavalia bonariensis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.3Å Ligands: CA, MMA, MN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function LECA_CANBN D-mannose-specific lectin.[REFERENCE:1] Publication Abstract from PubMed CaBo is a mannose/glucose-specific lectin purified from seeds of Canavalia bonariensis. In the present work, we report the CaBo crystal structure determined to atomic resolution in the presence of X-man, a specific ligand. Similar to the structural characteristics of other legume lectins, CaBo presented the jellyroll motif, a metal binding site occupied by calcium and manganese ions close to the carbohydrate-recognition domain (CRD). In vitro test of CaBo cytotoxicity against glioma cells demonstrated its ability to decrease the cellular viability and migration by induction of autophagy and cell death. Molecular docking simulations corroborate previous data indicating that the lectin's biological activities occur mostly through interactions with glycoproteins since the lectin interacted favorably with several N-glycans, especially those of the high-mannose type. Together, these results suggest that CaBo interacts with glycosylated cell targets and elicits a remarkable antiglioma activity. Canavalia bonariensis lectin: Molecular bases of glycoconjugates interaction and antiglioma potential.,Cavada BS, Silva MTL, Osterne VJS, Pinto-Junior VR, Nascimento APM, Wolin IAV, Heinrich IA, Nobre CAS, Moreira CG, Lossio CF, Rocha CRC, Martins JL, Nascimento KS, Leal RB Int J Biol Macromol. 2017 Aug 10. pii: S0141-8130(17)32351-6. doi:, 10.1016/j.ijbiomac.2017.08.023. PMID:28803976 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA