5uco

Benzophenone synthase from Hypericum androsaemum

spinqualitylabelsall models 5uco, resolution 2.85Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5uco is a 2 chain structure with sequence from Hypericum androsaemum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.85Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function TBSYN_HYPAN Type III polyketide synthase involved in the biosynthesis of benzophenones and xanthones. The preferred substrate is benzoyl-CoA, but can also use 3-hydroxybenzoyl-CoA with a lower activity. Publication Abstract from PubMed Biphenyl synthase and benzophenone synthase constitute an evolutionarily distinct clade of type III polyketide synthases (PKSs) that use benzoic acid-derived substrates to produce defense metabolites in plants. The use of benzoyl-CoA as an endogenous substrate is unusual for type III PKSs. Moreover, sequence analyses indicate that the residues responsible for the functional diversification of type III PKSs are mutated in benzoic acid-specific type III PKSs. In order to gain a better understanding of structure-function relationships within the type III PKS family, the crystal structures of biphenyl synthase from Malus x domestica and benzophenone synthase from Hypericum androsaemum were compared with the structure of an archetypal type III PKS: chalcone synthase from Malus x domestica. Both biphenyl synthase and benzophenone synthase contain mutations that reshape their active-site cavities to prevent the binding of 4-coumaroyl-CoA and to favor the binding of small hydrophobic substrates. The active-site cavities of biphenyl synthase and benzophenone synthase also contain a novel pocket associated with their chain-elongation and cyclization reactions. Collectively, these results illuminate structural determinants of benzoic acid-specific type III PKSs and expand the understanding of the evolution of specialized metabolic pathways in plants. Molecular architectures of benzoic acid-specific type III polyketide synthases.,Stewart C Jr, Woods K, Macias G, Allan AC, Hellens RP, Noel JP Acta Crystallogr D Struct Biol. 2017 Dec 1;73(Pt 12):1007-1019. doi:, 10.1107/S2059798317016618. Epub 2017 Nov 30. PMID:29199980 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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