5v93

Cryo-EM structure of the 70S ribosome from Mycobacterium tuberculosis bound with Capreomycin

Loading... Status: waiting for Mol* initialization Structural highlights 5v93 is a 10 chain structure with sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 4Å Ligands: DPP, KBE, MYN, UAL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RS20_MYCTU Publication Abstract from PubMed Ribosomes from Mycobacterium tuberculosis (Mtb) possess species-specific ribosomal RNA (rRNA) expansion segments and ribosomal proteins (rProtein). Here, we present the near-atomic structures of the Mtb 50S ribosomal subunit and the complete Mtb 70S ribosome, solved by cryo-electron microscopy. Upon joining of the large and small ribosomal subunits, a 100-nt long expansion segment of the Mtb 23S rRNA, named H54a or the 'handle', switches interactions from with rRNA helix H68 and rProtein uL2 to with rProtein bS6, forming a new intersubunit bridge 'B9'. In Mtb 70S, bridge B9 is mostly maintained, leading to correlated motions among the handle, the L1 stalk and the small subunit in the rotated and non-rotated states. Two new protein densities were discovered near the decoding center and the peptidyl transferase center, respectively. These results provide a structural basis for studying translation in Mtb as well as developing new tuberculosis drugs. Structural insights into species-specific features of the ribosome from the human pathogen Mycobacterium tuberculosis.,Yang K, Chang JY, Cui Z, Li X, Meng R, Duan L, Thongchol J, Jakana J, Huwe CM, Sacchettini JC, Zhang J Nucleic Acids Res. 2017 Oct 13;45(18):10884-10894. doi: 10.1093/nar/gkx785. PMID:28977617 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosome 3D structures

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