5vyq | pdb_00005vyq

Crystal structure of the N-formyltransferase Rv3404c from mycobacterium tuberculosis in complex with YDP-Qui4N and folinic acid

spinqualitylabelsall models 5vyq, resolution 1.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5vyq is a 2 chain structure with sequence from Mycobacterium tuberculosis CAS/NITR204. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.6Å Ligands: 0FX, CL, EDO, EP1, FON, K, LI, NA, TYD Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function R4MIX2_MYCTX Publication Abstract from PubMed The causative agent of tuberculosis, Mycobacterium tuberculosis, is a bacterium with a complex cell wall and a complicated life cycle. The genome of M. tuberculosis contains well over 4000 genes thought to encode proteins. One of these codes for a putative enzyme referred to as Rv3404c, which has attracted research attention as a potential virulence factor for over 12 years. Here we demonstrate that Rv3404c functions as a sugar N-formyltransferase that converts dTDP-4-amino-4,6-dideoxyglucose into dTDP-4-formamido-4,6-dideoxyglucose using N10-formyltetrahydrofolate as the carbon source. Kinetic analyses demonstrate that Rv3404c displays a significant catalytic efficiency of 1.1 x 104 M-1 s-1. In addition, we report the X-ray structure of a ternary complex of Rv3404c solved in the presence of N5-formyltetrahydrofolate and dTDP-4-amino-4,6-dideoxyglucose. The final model of Rv3404c was refined to an overall R-factor of 16.8% at 1.6 A resolution. The results described herein are especially intriguing given that there have been no published reports of N-formylated sugars associated with M. tuberculosis. The data thus provide a new avenue of research into this fascinating, yet deadly, organism that apparently has been associated with human infection since ancient times. Biochemical Investigation of Rv3404c from Mycobacterium tuberculosis.,Dunsirn MM, Thoden JB, Gilbert M, Holden HM Biochemistry. 2017 Jul 25;56(29):3818-3825. doi: 10.1021/acs.biochem.7b00506., Epub 2017 Jul 14. PMID:28665588 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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