5wx9
Crystal Structure of AtERF96 with GCC-box
Structural highlights
FunctionERF96_ARATH Probably acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). Publication Abstract from PubMedThe phytohormone ethylene is widely involved in many developmental processes and is a crucial regulator of defense responses against biotic and abiotic stresses in plants. Ethylene-responsive element binding protein, a member of the APETALA2/ethylene response factor (AP2/ERF) superfamily, is a transcription factor that regulates stress-responsive genes by recognizing a specific cis-acting element of target DNA. A previous study showed only the NMR structure of the AP2/ERF domain of AtERF100 in complex with a GCC box DNA motif. In this report, we determined the crystal structure of AtERF96 in complex with a GCC box at atomic resolution. We analyzed the binding residues of the conserved AP2/ERF domain in the DNA recognition sequence. In addition to the AP2/ERF domain, an N-terminal alpha-helix of AtERF96 participates in DNA interaction in the flanking region. We also demonstrated the structure of AtERF96 EDLL motif, a unique conserved motif in the group IX of AP2/ERF family, might involve in the transactivation of defense-related genes. Our study establishes the structural basis of the AtERF96 transcription factor in complex with the GCC box, as well as the DNA binding mechanisms of the N-terminal alpha-helix and AP2/ERF domain. Structural insights into Arabidopsis ethylene response factor 96 with an extended N-terminal binding to GCC box.,Chen CY, Lin PH, Chen KH, Cheng YS Plant Mol Biol. 2020 Nov;104(4-5):483-498. doi: 10.1007/s11103-020-01052-5. Epub , 2020 Aug 19. PMID:32813232[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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