Structural highlights
Function
WHAMM_HUMAN Acts as a nucleation-promoting factor (NPF) that stimulates Arp2/3-mediated actin polymerization both at the Golgi apparatus and along tubular membranes. Its activity in membrane tubulation requires F-actin and interaction with microtubules. Proposed to use coordinated actin-nucleating and microtubule-binding activities of distinct WHAMM molecules to drive membrane tubule elongation; when MT-bound can recruit and remodel membrane vesicles but is prevented to activate the Arp2/3 complex. Involved as a regulator of Golgi positioning and morphology. Participates in vesicle transport between the reticulum endoplasmic and the Golgi complex. Required for RhoD-dependent actin reorganization such as in cell adhesion and cell migration.[1] [2] [3]
References
- ↑ Campellone KG, Webb NJ, Znameroski EA, Welch MD. WHAMM is an Arp2/3 complex activator that binds microtubules and functions in ER to Golgi transport. Cell. 2008 Jul 11;134(1):148-61. PMID:18614018 doi:https://dx.doi.org/S0092-8674(08)00693-4
- ↑ Shen QT, Hsiue PP, Sindelar CV, Welch MD, Campellone KG, Wang HW. Structural insights into WHAMM-mediated cytoskeletal coordination during membrane remodeling. J Cell Biol. 2012 Oct 1;199(1):111-24. doi: 10.1083/jcb.201204010. PMID:23027905 doi:https://dx.doi.org/10.1083/jcb.201204010
- ↑ Gad AK, Nehru V, Ruusala A, Aspenstrom P. RhoD regulates cytoskeletal dynamics via the actin nucleation-promoting factor WASp homologue associated with actin Golgi membranes and microtubules. Mol Biol Cell. 2012 Dec;23(24):4807-19. doi: 10.1091/mbc.E12-07-0555. Epub 2012, Oct 19. PMID:23087206 doi:https://dx.doi.org/10.1091/mbc.E12-07-0555
