5xgz

Metagenomic glucose-tolerant glycosidase

spinqualitylabelsall models 5xgz, resolution 1.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5xgz is a 2 chain structure with sequence from Uncultured microorganism. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.75Å Ligands: GOL, NI, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A1E1FFN6_9ZZZZ Publication Abstract from PubMed MeBglD2, a metagenomic beta-glycosidase, is stimulated by various saccharides, including D-glucose, D-xylose, and maltose, and it promotes the enzymatic saccharification of plant biomass. To improve the thermostability of MeBglD2, its X-ray crystal structure was analyzed, and the amino acid residues responsible for its thermostability were identified using the structural information. Mutations in His8, Asn59, and Gly295 improved the thermostability of MeBglD2, and the combination of these mutations resulted in the highest thermostability. Compared with wild-type MeBglD2, thermostable MeBglD2 mutants promoted plant biomass saccharification using Trichoderma reesei cellulase. In addition to thermostability, the thermostable mutants exhibited higher tolerance to ethanol, dimethyl sulfoxide, and copper ions, indicating that the MeBglD2 mutants generated in this study were improved in their tolerance to not only high temperature but also to organic solvents and metal ions. Improved thermostability of a metagenomic glucose-tolerant beta-glycosidase based on its X-ray crystal structure.,Matsuzawa T, Watanabe M, Yaoi K Appl Microbiol Biotechnol. 2017 Dec;101(23-24):8353-8363. doi:, 10.1007/s00253-017-8525-9. Epub 2017 Oct 23. PMID:29063172 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA