5xz0

Staphylococcal Enterotoxin B (SEB) mutant S19 - N23A, Y90A, R110A and F177A

spinqualitylabelsall models 5xz0, resolution 3.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5xz0 is a 2 chain structure with sequence from Staphylococcus aureus subsp. aureus COL. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.002Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A0H2WZB2_STAAC Publication Abstract from PubMed Four mutations (N23A, Y90A, R110A and F177A) were introduced into S19, a vaccine candidate for staphylococcal enterotoxin B (SEB), resulting in a lower binding affinity towards the T-cell receptor beta chain (TCB) and reducing its superantigen activity. The structure of S19 was solved and was superposed on the native or complex structure of SEB. In the superposition model, mutations that were introduced seemed to reduce the number of hydrogen bonds at the SEB-TCB interface. S19 also displayed an unexpected structural change around the flexible-loop region owing to the Y90A mutation. This local structural change provided evidence that the mutated form of S19 could have a lower affinity for major histocompatibility complex (MHC) class II than wild-type SEB. Structure of the staphylococcal enterotoxin B vaccine candidate S19 showing eliminated superantigen activity.,Jeong WH, Song DH, Hur GH, Jeong ST Acta Crystallogr F Struct Biol Commun. 2017 Nov 1;73(Pt 11):595-600. doi:, 10.1107/S2053230X17014844. Epub 2017 Oct 20. PMID:29095152 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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