5z7w

Crystal structure of Striga hermonthica HTL1 (ShHTL1)

spinqualitylabelsall models 5z7w, resolution 1.66Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5z7w is a 2 chain structure with sequence from Striga hermonthica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.657Å Ligands: GOL, MG, NA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A0M4AV81_STRHE Publication Abstract from PubMed HYPOSENSITIVE TO LIGHT (HTL) and DWARF14 (D14) mediate the perception of karrikin and strigolactone, which stimulates germination of the parasitic weed Striga. However, their role in parasitic seeds is poorly understood, and the basis for their differing responsiveness remains unclear. Here, we show that Striga hermonthica HTL proteins (ShHTLs) in 'conserved' and 'intermediate' clades are able to bind karrikin. The 'divergent' clade is able to hydrolyze strigolactone. Unexpectedly, we find that ShD14 is also capable of hydrolyzing strigolactone. Through comparative analysis of ShHTLs and ShD14 crystal structures, we provide insights into the basis for their selectivity. Moreover, we show that both ShD14 and divergent clade ShHTLs, but not conserved and intermediate clade ShHTLs, can interact with the putative downstream signaling component ShMAX2 in the presence of the synthetic strigolactone, rac-GR24. These findings provide insight into how strigolactone is perceived and how ligand specificity is determined. Structural analysis of HTL and D14 proteins reveals the basis for ligand selectivity in Striga.,Xu Y, Miyakawa T, Nosaki S, Nakamura A, Lyu Y, Nakamura H, Ohto U, Ishida H, Shimizu T, Asami T, Tanokura M Nat Commun. 2018 Sep 26;9(1):3947. doi: 10.1038/s41467-018-06452-2. PMID:30258184 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA