5zcw

Structure of the Methanosarcina mazei class II CPD-photolyase in complex with intact, phosphodiester linked, CPD-lesion

spinqualitylabelsall models 5zcw, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5zcw is a 6 chain structure with sequence from Methanosarcina mazei and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.7Å Ligands: ACT, FAD, SO4, TTD Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q8PYK9_METMA Publication Abstract from PubMed Cyclobutane pyrimidine dimer (CPD) photolyases harness the energy of blue light to repair UV-induced DNA CPDs. Upon binding, CPD photolyases cause the photodamage to flip out of the duplex DNA and into the catalytic site of the enzyme. This process, called base-flipping, induces a kink in the DNA, as well as an unpaired bubble, which are stabilized by a network of protein-nucleic acid interactions. Previously, several co-crystal structures have been reported in which the binding mode of CPD photolyases has been studied in detail. However, in all cases the internucleoside linkage of the photodamage site was a chemically synthesized formacetal analogue and not the natural phosphodiester. Here, the first crystal structure and conformational analysis via molecular-dynamics simulations of a class II CPD photolyase in complex with photodamaged DNA that contains a natural cyclobutane pyrimidine dimer with an intra-lesion phosphodiester linkage are presented. It is concluded that a highly conserved bubble-intruding region (BIR) mediates stabilization of the open form of CPD DNA when complexed with class II CPD photolyases. Twist and turn: a revised structural view on the unpaired bubble of class II CPD photolyase in complex with damaged DNA.,Maestre-Reyna M, Yamamoto J, Huang WC, Tsai MD, Essen LO, Bessho Y IUCrJ. 2018 Aug 8;5(Pt 5):608-618. doi: 10.1107/S205225251800996X. eCollection, 2018 Sep 1. PMID:30224964 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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