5zs6

Dodecameric structure of a small Heat Shock Protein from Mycobacterium marinum M: Form-2

spinqualitylabelsall models 5zs6, resolution 2.81Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5zs6 is a 2 chain structure with sequence from Mycobacterium marinum M. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.8119135Å Ligands: CL, NA, PEG, PRO Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B2HF11_MYCMM Publication Abstract from PubMed Small heat shock proteins (sHSPs) are ATP-independent molecular chaperones present ubiquitously in all kingdoms of life. Their low molecular weight subunits associate to form higher order structures. Under conditions of stress, sHSPs prevent aggregation of substrate proteins by undergoing rapid changes in their conformation or stoichiometry. Polydispersity and dynamic nature of these proteins have made structural investigations through crystallography a daunting task. In pathogens like Mycobacteria, sHSPs are immuno-dominant antigens, enabling survival of the pathogen within the host and contributing to disease persistence. We characterized sHSPs from Mycobacterium marinum M and determined the crystal structure of one of these. The protein crystallized in three different conditions as dodecamers, with dimers arranged in a tetrahedral fashion to form a closed cage-like architecture. Interestingly, we found a pentapeptide bound to the dodecamers revealing one of the modes of sHSP-substrate interaction. Further, we have observed that ATP inhibits the chaperoning activity of the protein. Dodecameric structure of a small heat shock protein from Mycobacterium marinum M.,Bhandari S, Biswas S, Chaudhary A, Dutta S, Suguna K Proteins. 2019 Jan 11. doi: 10.1002/prot.25657. PMID:30632633 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Heat Shock Protein structures

Proteopedia Page Contributors and Editors (what is this?)

OCA