5zui

Crystal Structure of HSP104 from Chaetomium thermophilum

spinqualitylabelsall models 5zui, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5zui is a 1 chain structure with sequence from Chaetomium thermophilum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.701Å Ligands: ADP, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function G0S4G4_CHATD Publication Abstract from PubMed Hsp104 and its bacterial homolog ClpB form hexameric ring structures and mediate protein disaggregation. The disaggregated polypeptide is thought to thread through the central channel of the ring. However, the dynamic behavior of Hsp104 during disaggregation remains unclear. Here, we reported the stochastic conformational dynamics and a split conformation of Hsp104 disaggregase from Chaetomium thermophilum (CtHsp104) in the presence of ADP by X-ray crystallography, cryo-electron microscopy (EM), and high-speed atomic force microscopy (AFM). ADP-bound CtHsp104 assembles into a 65 left-handed spiral filament in the crystal structure at a resolution of 2.7 A. The unit of the filament is a hexamer of the split spiral structure. In the cryo-EM images, staggered and split hexameric rings were observed. Further, high-speed AFM observations showed that a substrate addition enhanced the conformational change and increased the split structure's frequency. Our data suggest that split conformation is an off-pathway state of CtHsp104 during disaggregation. Split conformation of Chaetomium thermophilum Hsp104 disaggregase.,Inoue Y, Hanazono Y, Noi K, Kawamoto A, Kimatsuka M, Harada R, Takeda K, Kita R, Iwamasa N, Shibata K, Noguchi K, Shigeta Y, Namba K, Ogura T, Miki K, Shinohara K, Yohda M Structure. 2021 Feb 25. pii: S0969-2126(21)00046-0. doi:, 10.1016/j.str.2021.02.002. PMID:33651974 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Heat Shock Protein structures

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