6a8h

Crystal structure of endo-arabinanase ABN-TS D27A mutant in complex with arabinotriose

spinqualitylabelsall models 6a8h, resolution 1.65Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6a8h is a 1 chain structure with sequence from Geobacillus thermodenitrificans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.65Å Ligands: AHR, CA, MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function IABN_GEOTD Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes the cleavage of endo alpha-(1->5)-L-arabinofuranosyl residues in debranched arabinan. Publication Abstract from PubMed The thermostable endo-1,5-alpha-L-arabinanase from Bacillus thermodenitrificans TS-3 (ABN-TS) hydrolyzes the alpha-1,5-L-arabinofuranoside linkages of arabinan. In this study, the crystal structures of inactive ABN-TS mutants, D27A and D147N, were determined in complex with arabino-oligosaccharides. The crystal structures revealed that ABN-TS has at least six subsites in the deep V-shaped cleft formed across one face of the propeller structure. The structural features indicate that substrate recognition is profoundly influenced by the remote subsites as well as by the subsites surrounding the active center. The `open' structure of the substrate-binding cleft of the endo-acting ABN-TS is suitable for the random binding of several sugar units in polymeric substrates. Structures of endo-1,5-alpha-L-arabinanase mutants from Bacillus thermodenitrificans TS-3 in complex with arabino-oligosaccharides.,Yamaguchi A, Sogabe Y, Fukuoka S, Sakai T, Tada T Acta Crystallogr F Struct Biol Commun. 2018 Dec 1;74(Pt 12):774-780. doi:, 10.1107/S2053230X18015947. Epub 2018 Nov 26. PMID:30511671 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Arabinanase 3D structures

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