6abw

Crystal structure of citrate synthase (Msed_0281) from Metallosphaera sedula in complex with acetyl-CoA

spinqualitylabelsall models 6abw, resolution 1.72Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6abw is a 1 chain structure with sequence from Metallosphaera sedula DSM 5348. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.72Å Ligands: ACO, GOL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A4YDF6_METS5 Publication Abstract from PubMed Metallosphaera sedula is a thermoacidophilic archaeon that has carbon fixation ability using the 3-hydroxypropionate/4-hydroxybutyrate(3-HP/4-HB) cycle, and has an incomplete TCA cycle to produce necessary biosynthetic precursors. The citrate synthase from M. sedula (MsCS) is an enzyme involved in the first step of the incomplete TCA cycle, catalyzing the conversion of oxaloacetate and acetyl-CoA into citrate and coenzyme A. To investigate the molecular mechanism of MsCS, we determined its crystal structure at 1.8A resolution. As other known CSs, MsCS functions as a dimer, and each monomer consists of two domains, a large domain and a small domain. We also determined the structure of the complex with acetyl-CoA and revealed the acetyl-CoA binding mode of MsCS. Structural comparison of MsCS with another CS in complex with oxaloacetate enabled us to predict the oxaloacetate binding site. Moreover, we performed inhibitory kinetic analyses of MsCS, and showed that the protein is inhibited by citrate and ATP by competitive and non-competitive inhibition modes, respectively, but not by NADH. Based on these results, we suggest that MsCS belongs to the type-I CS with structural and biochemical properties similar to those of CSs involved in the conventional TCA cycle. Crystal structure and biochemical properties of msed_0281, the citrate synthase from Metallosphaera sedula.,Lee SH, Kim KJ Biochem Biophys Res Commun. 2019 Jan 2. pii: S0006-291X(18)32860-2. doi:, 10.1016/j.bbrc.2018.12.172. PMID:30611567 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Citrate Synthase 3D structures

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