6abx

Crystal structure of citrate synthase (Msed_1522) from Metallosphaera sedula in complex with citrate

spinqualitylabelsall models 6abx, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6abx is a 2 chain structure with sequence from Metallosphaera sedula DSM 5348. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.7Å Ligands: FLC, GOL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A4YGX6_METS5 Publication Abstract from PubMed Metallosphaera sedula is a thermoacidophilic archaeon and has an incomplete TCA/glyoxylate cycle that is used for production of biosynthetic precursors of essential metabolites. Citrate synthase from M. sedula (MsCS) is an enzyme involved in the first step of the incomplete TCA/glyoxylate cycle by converting oxaloacetate and acetyl-CoA into citrate and coenzyme A. To elucidate the inhibition properties of MsCS, we determined its crystal structure at 1.7 A resolution. Like other Type-I CS, MsCS functions as a dimer and each monomer consists of two distinct domains, a large domain and a small domain. The oxaloacetate binding site locates at the cleft between the two domains, and the active site was more closed upon binding of the oxaloacetate substrate than binding of the citrate product. Interestingly, the inhibition kinetic analysis showed that, unlike other Type-I CSs, MsCS is non-competitively inhibited by NADH. Finally, amino acids and structural comparison of MsCS with other Type-II CSs, which were reported to be non-competitively inhibited by NADH, revealed that MsCS has quite unique NADH binding mode for non-competitive inhibition. Structural insights into the inhibition properties of archaeon citrate synthase from Metallosphaera sedula.,Lee SH, Son HF, Kim KJ PLoS One. 2019 Feb 22;14(2):e0212807. doi: 10.1371/journal.pone.0212807., eCollection 2019. PMID:30794680 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Citrate Synthase 3D structures

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