6b10

C. Jejuni Agmatine Deiminase

spinqualitylabelsall models 6b10, resolution 2.09Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6b10 is a 2 chain structure with sequence from Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.09Å Ligands: K, PO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q0P9V0_CAMJE Publication Abstract from PubMed Campylobacter jejuni is the most common bacterial cause of gastroenteritis and a major contributor to infant mortality in the developing world. The increasing incidence of antibiotic-resistant C. jejuni only adds to the urgency to develop effective therapies. Because of the essential role that polyamines play, particularly in protection from oxidative stress, enzymes involved in the biosynthesis of these metabolites are emerging as promising antibiotic targets. The recent description of an alternative pathway for polyamine synthesis, distinct from that in human cells, in C. jejuni, suggests this pathway could be a target for novel therapies. To that end, we determined X-ray crystal structures of C. jejuni agmatine deiminase (CjADI) and demonstrate that loss of CjADI function contributes to antibiotic sensitivity, likely due to polyamine starvation. The structures provide details of key molecular features of the active site of this protein. Comparison of the unliganded structure (2.1 A resolution) to that of the CjADI-agmatine complex (2.5 A) reveals significant structural rearrangements that occur upon substrate binding. The shift of two helical regions of the protein and a large conformational change in a loop near the active site generates a narrow binding pocket around the bound substrate. This change optimally positions the substrate for catalysis. In addition, kinetic analysis of this enzyme demonstrates that CjADI is an iminohydrolase that effectively deiminates agmatine. Our data suggest that C. jejuni agmatine deiminase is a potentially important target to combat antibiotic resistance, and these results provide a valuable framework to guide future drug development. Structural and functional basis for targeting Campylobacter jejuni agmatine deiminase to overcome antibiotic resistance.,Shek R, Dattmore DA, Stives DP, Jackson AL, Chatfield CH, Hicks KA, French JB Biochemistry. 2017 Nov 30. doi: 10.1021/acs.biochem.7b00982. PMID:29190068 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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