6bo4
Open state structure of the full-length TRPV2 cation channel with a resolved pore turret domain
Structural highlights
FunctionTRPV2_RAT Calcium-permeable, non-selective cation channel with an outward rectification. Seems to be regulated, at least in part, by growth factors, like IGF1, PDGF and morphogenetic neuropeptide/head activator. May transduce physical stimuli in mast cells. Activated by temperatures higher than 52 degrees Celsius; is not activated by vanilloids and acidic pH (By similarity).[1] [2] Publication Abstract from PubMedCation channels of the transient receptor potential (TRP) family serve important physiological roles by opening in response to diverse intra- and extracellular stimuli that regulate their lower or upper gates. Despite extensive studies, the mechanism coupling these gates has remained obscure. Previous structures have failed to resolve extracellular loops, known in the TRPV subfamily as 'pore turrets', which are proximal to the upper gates. We established the importance of the pore turret through activity assays and by solving structures of rat TRPV2, both with and without an intact turret at resolutions of 4.0 A and 3.6 A, respectively. These structures resolve the full-length pore turret and reveal fully open and partially open states of TRPV2, both with unoccupied vanilloid pockets. Our results suggest a mechanism by which physiological signals, such as lipid binding, can regulate the lower gate and couple to the upper gate through a pore-turret-facilitated mechanism. Structures of TRPV2 in distinct conformations provide insight into role of the pore turret.,Dosey TL, Wang Z, Fan G, Zhang Z, Serysheva II, Chiu W, Wensel TG Nat Struct Mol Biol. 2019 Jan;26(1):40-49. doi: 10.1038/s41594-018-0168-8. Epub, 2018 Dec 31. PMID:30598551[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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