6brd

Crystal structure of rifampin monooxygenase from Streptomyces venezuelae, complexed with rifampin and FAD

spinqualitylabelsall models 6brd, resolution 3.32Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6brd is a 3 chain structure with sequence from Streptomyces venezuelae ATCC 10712. This structure supersedes the now removed PDB entry 5vqc. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.32Å Ligands: CL, FAD, MG, RFP Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function ROX_STRVP Monooxygenase that can modify rifampicin, thereby inactivating its antibiotic activity (PubMed:29398560). Inactivates a broad range of rifamycin antibiotics (PubMed:29398560). Publication Abstract from PubMed Rifamycin monooxygenases (Rox) are present in a variety of environmental bacteria and are associated with decomposition of the clinically utilized antibiotic rifampin. Here we report the structure and function of a drug-inducible rox gene from Streptomyces venezuelae, which encodes a class A flavoprotein monooxygenase that inactivates a broad range of rifamycin antibiotics. Our findings describe a mechanism of rifamycin inactivation initiated by monooxygenation of the 2-position of the naphthyl group, which subsequently results in ring opening and linearization of the antibiotic. The result is an antibiotic that no longer adopts the basket-like structure essential for binding to the RNA exit tunnel of the target RpoB, thereby providing the molecular logic of resistance. This unique mechanism of enzymatic inactivation underpins the broad spectrum of rifamycin resistance mediated by Rox enzymes and presents a new antibiotic resistance mechanism not yet seen in microbial antibiotic detoxification. Rox, a Rifamycin Resistance Enzyme with an Unprecedented Mechanism of Action.,Koteva K, Cox G, Kelso JK, Surette MD, Zubyk HL, Ejim L, Stogios P, Savchenko A, Sorensen D, Wright GD Cell Chem Biol. 2018 Jan 26. pii: S2451-9456(18)30031-X. doi:, 10.1016/j.chembiol.2018.01.009. PMID:29398560 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Monooxygenase 3D structures

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