6by4

Single-State 14-mer UUCG Tetraloop calculated from Exact NOEs

spinqualitylabelsall models 6by4 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6by4 is a 1 chain structure with sequence from Synthetic construct. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed RNA not only translates the genetic code into proteins, but also carries out important cellular functions. Understanding such functions requires knowledge of the structure and dynamics at atomic resolution. Almost half of the published RNA structures have been solved by nuclear magnetic resonance (NMR). However, as a result of severe resonance overlap and low proton density, high-resolution RNA structures are rarely obtained from nuclear Overhauser enhancement (NOE) data alone. Instead, additional semi-empirical restraints and labor-intensive techniques are required for structural averages, while there are only a few experimentally derived ensembles representing dynamics. Here we show that our exact NOE (eNOE) based structure determination protocol is able to define a 14-mer UUCG tetraloop structure at high resolution without other restraints. Additionally, we use eNOEs to calculate a two-state structure, which samples its conformational space. The protocol may open an avenue to obtain high-resolution structures of small RNA of unprecedented accuracy with moderate experimental efforts. High-resolution small RNA structures from exact nuclear Overhauser enhancement measurements without additional restraints.,Nichols PJ, Henen MA, Born A, Strotz D, Guntert P, Vogeli B Commun Biol. 2018 Jun 7;1:61. doi: 10.1038/s42003-018-0067-x. eCollection 2018. PMID:30271943 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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