6c7s

Structure of Rifampicin Monooxygenase with Product Bound

spinqualitylabelsall models 6c7s, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6c7s is a 1 chain structure with sequence from Nocardia farcinica IFM 10152. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.1Å Ligands: EDO, FAD, RFH, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function ROX_NOCFA Monooxygenase that can modify rifampicin, thereby inactivating its antibiotic activity (PubMed:19942945, PubMed:29578336). It constitutes a secondary rifampicin resistance factor (PubMed:19942945). Publication Abstract from PubMed Rifampicin monooxygenase (RIFMO) decreases the potency of rifampicin (RIF) by converting it to oxidative products. Further decomposition of RIF has been observed in bacteria producing RIFMO and contributes to RIFMO-mediated drug resistance. Here we report the first crystal structure of RIFMO in complex with the hydroxylated RIF product. The 2.10 A resolution structure reveals a breach of the ansa aliphatic chain of RIF between naphthoquinone C2 and amide N1. Our data suggest that RIFMO catalyzes the hydroxylation of RIF at the C2 atom followed by cleavage of the ansa linkage, which leads to inactivation of the antibiotic by preventing key contacts with the RNA polymerase target. Structural Evidence for Rifampicin Monooxygenase Inactivating Rifampicin by Cleaving Its Ansa-Bridge.,Liu LK, Dai Y, Abdelwahab H, Sobrado P, Tanner JJ Biochemistry. 2018 Apr 10;57(14):2065-2068. doi: 10.1021/acs.biochem.8b00190., Epub 2018 Mar 30. PMID:29578336 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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