6cih

Crystal structure of a group II intron lariat in the post-catalytic state

spinqualitylabelsall models 6cih, resolution 3.68Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6cih is a 2 chain structure with sequence from Pylaiella littoralis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.676Å Ligands: IRI, MG, NA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The group II intron and the spliceosome share a common active site architecture and are thought to be evolutionarily related. Here we report the 3.7 A crystal structure of a eukaryotic group II intron in the lariat-3' exon form, immediately preceding the second step of splicing, analogous to the spliceosomal P complex. This structure reveals the location of the intact 3' splice site within the catalytic core of the group II intron. The 3'-OH of the 5' exon is positioned in close proximity to the 3' splice site for nucleophilic attack and exon ligation. The active site undergoes conformational rearrangements with the catalytic triplex having different configurations before and after the second step of splicing. We describe a complete model for the second step of group II intron splicing that incorporates a dynamic catalytic triplex being responsible for creating the binding pocket for 3' splice site capture. Structural basis for the second step of group II intron splicing.,Chan RT, Peters JK, Robart AR, Wiryaman T, Rajashankar KR, Toor N Nat Commun. 2018 Nov 8;9(1):4676. doi: 10.1038/s41467-018-06678-0. PMID:30410046 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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