6cmk

Crystal structure of Citrobacter koseri AztD

spinqualitylabelsall models 6cmk, resolution 1.73Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6cmk is a 2 chain structure with sequence from Citk8. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: EDO, MES, PEG, PGE, ZN Gene: CKO_00948 (CITK8) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Zinc acquisition from limited environments is critical for bacterial survival and pathogenesis. AztD has been identified as a periplasmic or cell surface zinc-binding protein in numerous bacterial species. In Paracoccus denitrificans, AztD can transfer zinc directly to AztC, the solute binding protein for a zinc-specific ATP-binding cassette transporter system, suggesting a role in zinc acquisition and homeostasis. Here, we present the first cry stal structures of AztD from P. denitrificans and tbe human pathogen Citrobacter koseri, revealing a beta-propeller fold and two high-affinity zinc-binding sites that are highly conserved among AztD homologs. These structures combined with transfer assays using WT and mutant proteins provide rare insight into the mechanism of direct zinc transfer from one protein to another. Given the importance of zinc import to bacterial pathogenesis, these insights may prove valuable to the development of zinc transfer inhibitors as antibiotics. Crystal structures of AztD provide mechanistic insights into direct zinc transfer between proteins.,Neupane DP, Fullam SH, Chacon KN, Yukl ET Commun Biol. 2019 Aug 9;2:308. doi: 10.1038/s42003-019-0542-z. eCollection 2019. PMID:31428696 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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