6cpv

MicroED structure of NaK ion channel reveals a process of Na+ partition into the selectivity filter

spinqualitylabelsall models 6cpv, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6cpv is a 2 chain structure with sequence from Bacillus cereus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron crystallography, Resolution 2.5Å Ligands: MPD, NA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q81HW2_BACCR Publication Abstract from PubMed Sodium (Na(+)) is a ubiquitous and important inorganic salt mediating many critical biological processes such as neuronal excitation, signaling, and facilitation of various transporters. The hydration states of Na(+) are proposed to play critical roles in determining the conductance and the selectivity of Na(+) channels, yet they are rarely captured by conventional structural biology means. Here we use the emerging cryo-electron microscopy (cryoEM) method micro-electron diffraction (MicroED) to study the structure of a prototypical tetrameric Na(+)-conducting channel, NaK, to 2.5 A resolution from nano-crystals. Two new conformations at the external site of NaK are identified, allowing us to visualize a partially hydrated Na(+) ion at the entrance of the channel pore. A process of dilation coupled with Na(+) movement is identified leading to valuable insights into the mechanism of ion conduction and gating. This study lays the ground work for future studies using MicroED in membrane protein biophysics. MicroED structure of the NaK ion channel reveals a Na(+) partition process into the selectivity filter.,Liu S, Gonen T Commun Biol. 2018;1:38. doi: 10.1038/s42003-018-0040-8. Epub 2018 May 3. PMID:30167468 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Potassium channel 3D structures

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