6ds1

Crystal structure of Cj0485 dehydrogenase in complex with NADP+

spinqualitylabelsall models 6ds1, resolution 2.12Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6ds1 is a 4 chain structure with sequence from Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.119Å Ligands: GOL, MG, NAP Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q0PB28_CAMJE Publication Abstract from PubMed Although the gastrointestinal pathogen Campylobacter jejuni was considered asaccharolytic, >50% of sequenced isolates possess an operon for L-fucose utilization. In C. jejuni NCTC11168, this pathway confers L-fucose chemotaxis and competitive colonization advantages in the piglet diarrhea model, but the catabolic steps remain unknown. Here we solved the putative dehydrogenase structure, resembling FabG of Burkholderia multivorans. The C. jejuni enzyme, FucX, reduces L-fucose and D-arabinose in vitro and both sugars are catabolized by fuc-operon encoded enzymes. This enzyme alone confers chemotaxis to both sugars in a non-carbohydrate-utilizing C. jejuni strain. Although C. jejuni lacks fucosidases, the organism exhibits enhanced growth in vitro when co-cultured with Bacteroides vulgatus, suggesting scavenging may occur. Yet, when excess amino acids are available, C. jejuni prefers them to carbohydrates, indicating a metabolic hierarchy exists. Overall this study increases understanding of nutrient metabolism by this pathogen, and identifies interactions with other gut microbes. The gastrointestinal pathogen Campylobacter jejuni metabolizes sugars with potential help from commensal Bacteroides vulgatus.,Garber JM, Nothaft H, Pluvinage B, Stahl M, Bian X, Porfirio S, Enriquez A, Butcher J, Huang H, Glushka J, Line E, Gerlt JA, Azadi P, Stintzi A, Boraston AB, Szymanski CM Commun Biol. 2020 Jan 7;3(1):2. doi: 10.1038/s42003-019-0727-5. PMID:31925306 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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