6e4d

Atomic structure of Mycobacterium tuberculosis DppA

spinqualitylabelsall models 6e4d, resolution 1.25Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6e4d is a 2 chain structure with sequence from Escherichia coli and Mycobacterium tuberculosis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.252Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function I6X811_MYCTU Publication Abstract from PubMed Iron is essential for growth of Mycobacterium tuberculosis (Mtb), but most iron in the human body is stored in heme within hemoglobin. Here, we demonstrate that the substrate-binding protein DppA of the inner membrane Dpp transporter is required for heme and hemoglobin utilization by Mtb. The 1.27 A crystal structure of DppA shows a tetrapeptide bound in the protein core and a large solvent-exposed crevice for heme binding. Mutation of arginine 179 in this cleft eliminates heme binding to DppA and prevents heme utilization by Mtb. The outer membrane proteins PPE36 and PPE62 are also required for heme and hemoglobin utilization, indicating that these pathways converge at the cell surface of Mtb. Albumin, the most abundant blood protein, binds heme specifically and bypasses the requirements for PPE36, PPE62 and Dpp. Thus, our study reveals albumin-dependent and -independent heme uptake pathways, highlighting the importance of iron acquisition from heme for Mtb. Heme and hemoglobin utilization by Mycobacterium tuberculosis.,Mitra A, Ko YH, Cingolani G, Niederweis M Nat Commun. 2019 Sep 18;10(1):4260. doi: 10.1038/s41467-019-12109-5. PMID:31534126 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also ABC transporter 3D structures

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