6evu

Adhesin domain of PrgB from Enterococcus faecalis

spinqualitylabelsall models 6evu, resolution 1.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6evu is a 1 chain structure with sequence from Enterococcus faecalis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.598Å Ligands: PO4, TRS Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q04112_ENTFL Publication Abstract from PubMed Gram-positive bacteria deploy type IV secretion systems (T4SSs) to facilitate horizontal gene transfer. The T4SSs of Gram-positive bacteria rely on surface adhesins as opposed to conjugative pili to facilitate mating. Enterococcus faecalis PrgB is a surface adhesin that promotes mating pair formation and robust biofilm development in an extracellular DNA (eDNA) dependent manner. Here we report the structure of the adhesin domain of PrgB. The adhesin domain binds and compacts DNA in vitro. In vivo PrgB deleted of its adhesin domain does not support cellular aggregation, biofilm development and conjugative DNA transfer. PrgB also binds lipoteichoic acid (LTA), which competes with DNA binding. We propose that PrgB binding and compaction of eDNA facilitates cell aggregation and plays an important role in establishment of early biofilms in mono- or polyspecies settings. Within these biofilms, PrgB mediates formation and stabilization of direct cell-cell contacts through alternative binding of cell-bound LTA, which in turn promotes establishment of productive mating junctions and efficient intra- or interspecies T4SS-mediated gene transfer. This article is protected by copyright. All rights reserved. PrgB Promotes Aggregation, Biofilm Formation, and Conjugation through DNA Binding and Compaction.,Schmitt A, Jiang K, Camacho MI, Jonna VR, Hofer A, Westerlund F, Christie PJ, Berntsson RP Mol Microbiol. 2018 May 3. doi: 10.1111/mmi.13980. PMID:29723434 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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