6exn

Post-catalytic P complex spliceosome with 3' splice site docked

Loading... Status: waiting for Mol* initialization Structural highlights 6exn is a 12 chain structure with sequence from Saccharomyces cerevisiae S288C. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.7Å Ligands: GTP, IHP, MG, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function PRP46_YEAST Involved in pre-mRNA splicing. May also be required for cell cycle progression at G2/M (By similarity). Publication Abstract from PubMed Introns are removed from eukaryotic messenger RNA precursors by the spliceosome in two transesterification reactions-branching and exon ligation. The mechanism of 3'-splice site recognition during exon ligation has remained unclear. Here we present the 3.7-angstrom cryo-electron microscopy structure of the yeast P-complex spliceosome immediately after exon ligation. The 3'-splice site AG dinucleotide is recognized through non-Watson-Crick pairing with the 5' splice site and the branch-point adenosine. After the branching reaction, protein factors work together to remodel the spliceosome and stabilize a conformation competent for 3'-splice site docking, thereby promoting exon ligation. The structure accounts for the strict conservation of the GU and AG dinucleotides at the 5' and 3' ends of introns and provides insight into the catalytic mechanism of exon ligation. Postcatalytic spliceosome structure reveals mechanism of 3'-splice site selection.,Wilkinson ME, Fica SM, Galej WP, Norman CM, Newman AJ, Nagai K Science. 2017 Dec 8;358(6368):1283-1288. doi: 10.1126/science.aar3729. Epub 2017 , Nov 16. PMID:29146871 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Nucleoprotein 3D structures Pre-mRNA splicing factors 3D structures Sm-like protein 3D structures

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