6eyx

Anti-CRISPR AcrIIa6 tetragonal form

spinqualitylabelsall models 6eyx, resolution 1.96Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6eyx is a 2 chain structure with sequence from Streptococcus virus DT1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.96Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q9XJC1_9CAUD Publication Abstract from PubMed CRISPR-Cas systems are bacterial anti-viral systems, and bacterial viruses (bacteriophages, phages) can carry anti-CRISPR (Acr) proteins to evade that immunity. Acrs can also fine-tune the activity of CRISPR-based genome-editing tools. While Acrs are prevalent in phages capable of lying dormant in a CRISPR-carrying host, their orthologs have been observed only infrequently in virulent phages. Here we identify AcrIIA6, an Acr encoded in 33% of virulent Streptococcus thermophilus phage genomes. The X-ray structure of AcrIIA6 displays some features unique to this Acr family. We compare the activity of AcrIIA6 to those of other Acrs, including AcrIIA5 (also from S. thermophilus phages), and characterize their effectiveness against a range of CRISPR-Cas systems. Finally, we demonstrate that both Acr families from S. thermophilus phages inhibit Cas9-mediated genome editing of human cells. Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins.,Hynes AP, Rousseau GM, Agudelo D, Goulet A, Amigues B, Loehr J, Romero DA, Fremaux C, Horvath P, Doyon Y, Cambillau C, Moineau S Nat Commun. 2018 Jul 25;9(1):2919. doi: 10.1038/s41467-018-05092-w. PMID:30046034 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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