6f0t

Crystal structure of Pizza6-SFW

spinqualitylabelsall models 6f0t, resolution 1.74Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6f0t is a 3 chain structure with sequence from Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.74Å Ligands: GOL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed beta-propeller proteins are highly symmetrical, being composed of a repeated motif with four anti-parallel beta-sheets arranged around a central axis. Recently we designed the first completely symmetrical beta-propeller protein, Pizza6, consisting of six identical tandem repeats. Pizza6 is expected to prove a useful building block for bionanotechnology, and also a tool to investigate the folding and evolution of beta-propeller proteins. Folding studies are made difficult by the high stability and the lack of buried Trp residues to act as monitor fluorophores, so we have designed and characterized several Trp-containing Pizza6 derivatives. In total four proteins were designed, of which three could be purified and characterized. Crystal structures confirm these mutant proteins maintain the expected structure, and a clear redshift of Trp fluorescence emission could be observed upon denaturation. Among the derivative proteins, Pizza6-AYW appears to be the most suitable model protein for future folding/unfolding kinetics studies as it has a comparable stability as natural beta-propeller proteins. Design of tryptophan-containing mutants of the symmetrical Pizza protein for biophysical studies.,Noguchi H, Mylemans B, De Zitter E, Van Meervelt L, Tame JRH, Voet A Biochem Biophys Res Commun. 2018 Mar 18;497(4):1038-1042. doi:, 10.1016/j.bbrc.2018.02.168. Epub 2018 Feb 23. PMID:29481797 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA