6fny

CRYSTAL STRUCTURE OF A CHOLINE SULFATASE FROM SINORHIZOBIUM MELLILOTI

spinqualitylabelsall models 6fny, resolution 2.79Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6fny is a 8 chain structure with sequence from Sinorhizobium meliloti 1021. This structure supersedes the now removed PDB entry 4ug4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CA, DDZ Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function BETC_RHIME Converts choline-O-sulfate into choline. Publication Abstract from PubMed Hydrolysis of organic sulfate esters proceeds by two distinct mechanisms, water attacking at either sulfur (S-O bond cleavage) or carbon (C-O bond cleavage). In primary and secondary alkyl sulfates attack at carbon is favored, whereas in aromatic sulfates and sulfated sugars attack at sulfur is preferred. This mechanistic distinction is mirrored in the classification of enzymes that catalyze sulfate ester hydrolysis: arylsulfatases catalyze S-O cleavage in sulfate sugars and arylsulfates and alkyl sulfatases break the C-O bond of alkyl sulfates. Sinorhizobium meliloti choline sulfatase (SmCS) efficiently catalyzes the hydrolysis of alkyl sulfate choline-O-sulfate (kcat/KM=4.8x10(3)s(-1)M(-1)) as well as arylsulfate 4-nitrophenyl sulfate (kcat/KM=12s(-1)M(-1)). Its 2.8A resolution X-ray structure shows a buried, largely hydrophobic active site in which a conserved glutamate (Glu386) plays a role in recognition of the quaternary ammonium group of the choline substrate. SmCS structurally resembles members of the alkaline phosphatase (AP) superfamily, being most closely related to dimeric arylsulfatases (ASs) and tetrameric phosphonate monoester hydrolases (PMHs). Although >70% of the amino acids between protomers align structurally (RMSDs 1.79-1.99A), the oligomeric structures show distinctly different packing and protomer-protomer interfaces. The latter also play an important role in active site formation. Mutagenesis of the conserved active site residues typical for arylsulfatases, H2(18)O-labeling studies and the observation of catalytically promiscuous behavior toward phosphoesters confirm the close relation to AP superfamily members and suggest that SmCS is an arylsulfatase that catalyzes S-O cleavage in alkyl sulfate esters with extreme catalytic proficiency. Structural and Mechanistic Analysis of the Choline Sulfatase from Sinorhizobium melliloti: A Class I Sulfatase Specific for an Alkyl Sulfate Ester.,van Loo B, Schober M, Valkov E, Heberlein M, Bornberg-Bauer E, Faber K, Hyvonen M, Hollfelder F J Mol Biol. 2018 Feb 16. pii: S0022-2836(18)30081-0. doi:, 10.1016/j.jmb.2018.02.010. PMID:29458126 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Sulfatase 3D structures

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