6frk

Structure of a prehandover mammalian ribosomal SRP and SRP receptor targeting complex

Loading... Status: waiting for Mol* initialization Structural highlights 6frk is a 10 chain structure with sequence from Canis lupus familiaris and Oryctolagus cuniculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.7Å Ligands: GNP, GTP, MG, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function SRP09_CANLF Publication Abstract from PubMed Signal recognition particle (SRP) targets proteins to the endoplasmic reticulum (ER). SRP recognizes the ribosome synthesizing a signal sequence and delivers it to the SRP receptor (SR) on the ER membrane followed by the transfer of the signal sequence to the translocon. Here, we present the cryo-EM structure of the mammalian translating ribosome in complex with SRP and SR in a conformation preceding signal sequence handover. The structure visualizes all eukaryotic-specific SRP and SR proteins and reveals their roles in stabilizing this conformation by forming a large protein assembly at the distal site of SRP RNA. We provide biochemical evidence that the GTP hydrolysis of SRP*SR is delayed at this stage, possibly to provide a time window for signal sequence handover to the translocon. Structure of a prehandover mammalian ribosomal SRP*SRP receptor targeting complex.,Kobayashi K, Jomaa A, Lee JH, Chandrasekar S, Boehringer D, Shan SO, Ban N Science. 2018 Mar 22. pii: science.aar7924. doi: 10.1126/science.aar7924. PMID:29567807 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosome 3D structures

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