6fuw

Cryo-EM structure of the human CPSF160-WDR33-CPSF30 complex bound to the PAS AAUAAA motif at 3.1 Angstrom resolution

Loading... Status: waiting for Mol* initialization Structural highlights 6fuw is a 4 chain structure with sequence from Homo sapiens and Unidentified adenovirus. This structure supersedes the now removed PDB entry 6fbs. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CPSF1_HUMAN Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction. Publication Abstract from PubMed Mammalian mRNA biogenesis requires specific recognition of a hexanucleotide AAUAAA motif in the polyadenylation signals (PAS) of precursor mRNA (pre-mRNA) transcripts by the cleavage and polyadenylation specificity factor (CPSF) complex. Here we present a 3.1-A-resolution cryo-EM structure of a core CPSF module bound to the PAS hexamer motif. The structure reveals the molecular interactions responsible for base-specific recognition, providing a rationale for mechanistic differences between mammalian and yeast 3' polyadenylation. Structural basis of AAUAAA polyadenylation signal recognition by the human CPSF complex.,Clerici M, Faini M, Muckenfuss LM, Aebersold R, Jinek M Nat Struct Mol Biol. 2018 Jan 22. pii: 10.1038/s41594-017-0020-6. doi:, 10.1038/s41594-017-0020-6. PMID:29358758 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also WD-repeat protein 3D structures

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