6gux

Dark-adapted structure of Archaerhodopsin-3 at 100K

spinqualitylabelsall models 6gux, resolution 1.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6gux is a 1 chain structure with sequence from Halorubrum sodomense. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.3Å Ligands: CA, CL, D12, DD9, NA, PCA, R16, RET Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function BACR3_HALSD Light-driven proton pump. Publication Abstract from PubMed Many transmembrane receptors have a desensitized state, in which they are unable to respond to external stimuli. The family of microbial rhodopsin proteins includes one such group of receptors, whose inactive or dark-adapted (DA) state is established in the prolonged absence of light. Here, we present high-resolution crystal structures of the ground (light-adapted) and DA states of Archaerhodopsin-3 (AR3), solved to 1.1 A and 1.3 A resolution respectively. We observe significant differences between the two states in the dynamics of water molecules that are coupled via H-bonds to the retinal Schiff Base. Supporting QM/MM calculations reveal how the DA state permits a thermodynamic equilibrium between retinal isomers to be established, and how this same change is prevented in the ground state in the absence of light. We suggest that the different arrangement of internal water networks in AR3 is responsible for the faster photocycle kinetics compared to homologs. Structures of the archaerhodopsin-3 transporter reveal that disordering of internal water networks underpins receptor sensitization.,Bada Juarez JF, Judge PJ, Adam S, Axford D, Vinals J, Birch J, Kwan TOC, Hoi KK, Yen HY, Vial A, Milhiet PE, Robinson CV, Schapiro I, Moraes I, Watts A Nat Commun. 2021 Jan 27;12(1):629. doi: 10.1038/s41467-020-20596-0. PMID:33504778 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Bacteriorhodopsin 3D structures

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