6gv9

Structure of the type IV pilus from enterohemorrhagic Escherichia coli (EHEC)

Loading... Status: waiting for Mol* initialization Structural highlights 6gv9 is a 14 chain structure with sequence from Escherichia coli O157:H7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 8Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q8X974_ECO57 Publication Abstract from PubMed Bacterial type 4a pili are dynamic surface filaments that promote bacterial adherence, motility, and macromolecular transport. Their genes are highly conserved among enterobacteria and their expression in enterohemorrhagic Escherichia coli (EHEC) promotes adhesion to intestinal epithelia and pro-inflammatory signaling. To define the molecular basis of EHEC pilus assembly, we determined the structure of the periplasmic domain of its major subunit PpdD (PpdDp), a prototype of an enterobacterial pilin subfamily containing two disulfide bonds. The structure of PpdDp, determined by NMR, was then docked into the density envelope of purified EHEC pili obtained by cryoelectron microscopy (cryo-EM). Cryo-EM reconstruction of EHEC pili at approximately 8 A resolution revealed extremely high pilus flexibility correlating with a large extended region of the pilin stem. Systematic mutagenesis combined with functional and interaction analyses identified charged residues essential for pilus assembly. Structural information on exposed regions and interfaces between EHEC pilins is relevant for vaccine and drug discovery. Structure and Assembly of the Enterohemorrhagic Escherichia coli Type 4 Pilus.,Bardiaux B, de Amorim GC, Luna Rico A, Zheng W, Guilvout I, Jollivet C, Nilges M, Egelman EH, Izadi-Pruneyre N, Francetic O Structure. 2019 Apr 9. pii: S0969-2126(19)30117-0. doi:, 10.1016/j.str.2019.03.021. PMID:31056419 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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