6ha4

Crystal structure of PAF - p-sulfonatocalix[4]arene complex

spinqualitylabelsall models 6ha4, resolution 1.33Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6ha4 is a 1 chain structure with sequence from Penicillium rubens Wisconsin 54-1255. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.33Å Ligands: GOL, T3Y Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B6HWK0_PENRW Publication Abstract from PubMed Synthetic macrocycles such as calixarenes and cucurbiturils are increasingly applied as mediators of protein assembly and crystallization. The macrocycle can facilitate assembly by providing a surface on which two or more proteins bind simultaneously. This work explores the capacity of the sulfonato-calix[n]arene (sclx n ) series to effect crystallization of PAF, a small, cationic antifungal protein. Co-crystallization with sclx4, sclx6 or sclx8 led to high-resolution crystal structures. In the absence of sclx n , diffraction-quality crystals of PAF were not obtained. Interestingly, all three sclx n were bound to a similar patch on PAF. The largest and most flexible variant, sclx8, yielded a dimer of PAF. Complex formation was evident in solution via NMR and ITC experiments, showing more pronounced effects with increasing macrocycle size. In agreement with the crystal structure, the ITC data suggested that sclx8 acts as a bidentate ligand. The contributions of calixarene size/conformation to protein recognition and assembly are discussed. Finally, it is suggested that the conserved binding site for anionic calixarenes implicates this region of PAF in membrane binding, which is a prerequisite for antifungal activity. Calixarene-mediated assembly of a small antifungal protein.,Alex JM, Rennie ML, Engilberge S, Lehoczki G, Dorottya H, Fizil A, Batta G, Crowley PB IUCrJ. 2019 Feb 5;6(Pt 2):238-247. doi: 10.1107/S2052252519000411. eCollection, 2019 Mar 1. PMID:30867921 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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