6hmj

Structure of an RNA-binding Light-Oxygen-Voltage Receptor

spinqualitylabelsall models 6hmj, resolution 2.51Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6hmj is a 4 chain structure with sequence from Microsphaera multipartita. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: EDO, FMN, GOL, IMD Gene: Namu_4361 (Microsphaera multipartita) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Sensory photoreceptor proteins underpin light-dependent adaptations in nature and enable the optogenetic control of organismal behavior and physiology. We identified the bacterial light-oxygen-voltage (LOV) photoreceptor PAL that sequence-specifically binds short RNA stem loops with around 20 nM affinity in blue light and weaker than 1 microM in darkness. A crystal structure rationalizes the unusual receptor architecture of PAL with C-terminal LOV photosensor and N-terminal effector units. The light-activated PAL-RNA interaction can be harnessed to regulate gene expression at the RNA level as a function of light in both bacteria and mammalian cells. The present results elucidate a new signal-transduction paradigm in LOV receptors and conjoin RNA biology with optogenetic regulation, thereby paving the way toward hitherto inaccessible optoribogenetic modalities. A blue light receptor that mediates RNA binding and translational regulation.,Weber AM, Kaiser J, Ziegler T, Pilsl S, Renzl C, Sixt L, Pietruschka G, Moniot S, Kakoti A, Juraschitz M, Schrottke S, Lledo Bryant L, Steegborn C, Bittl R, Mayer G, Moglich A Nat Chem Biol. 2019 Nov;15(11):1085-1092. doi: 10.1038/s41589-019-0346-y. Epub, 2019 Aug 26. PMID:31451761 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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