Structural highlights
6hqv is a 2 chain structure with sequence from Chatd. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | 3DS, GLU, NAD, PO4, SKM, ZN |
| NonStd Res: | MSE |
| Gene: | CTHT_0008860 (CHATD) |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[G0S061_CHATD] The AROM polypeptide catalyzes 5 consecutive enzymatic reactions in prechorismate polyaromatic amino acid biosynthesis.[HAMAP-Rule:MF_03143][PIRNR:PIRNR000514][SAAS:SAAS00712173]
Publication Abstract from PubMed
The AROM complex is a multifunctional metabolic machine with ten enzymatic domains catalyzing the five central steps of the shikimate pathway in fungi and protists. We determined its crystal structure and catalytic behavior, and elucidated its conformational space using a combination of experimental and computational approaches. We derived this space in an elementary approach, exploiting an abundance of conformational information from its monofunctional homologs in the Protein Data Bank. It demonstrates how AROM is optimized for spatial compactness while allowing for unrestricted conformational transitions and a decoupled functioning of its individual enzymatic entities. With this architecture, AROM poses a tractable test case for the effects of active site proximity on the efficiency of both natural metabolic systems and biotechnological pathway optimization approaches. We show that a mere colocalization of enzymes is not sufficient to yield a detectable improvement of metabolic throughput.
Architecture and functional dynamics of the pentafunctional AROM complex.,Arora Veraszto H, Logotheti M, Albrecht R, Leitner A, Zhu H, Hartmann MD Nat Chem Biol. 2020 Jul 6. pii: 10.1038/s41589-020-0587-9. doi:, 10.1038/s41589-020-0587-9. PMID:32632294[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Arora Veraszto H, Logotheti M, Albrecht R, Leitner A, Zhu H, Hartmann MD. Architecture and functional dynamics of the pentafunctional AROM complex. Nat Chem Biol. 2020 Jul 6. pii: 10.1038/s41589-020-0587-9. doi:, 10.1038/s41589-020-0587-9. PMID:32632294 doi:https://dx.doi.org/10.1038/s41589-020-0587-9
