6hqz

Crystal structure of the type III effector protein AvrRpt2 from Erwinia amylovora, a C70 family cysteine protease

spinqualitylabelsall models 6hqz, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6hqz is a 2 chain structure with sequence from Erwinia amylovora. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.8Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A2U7NR52_ERWAM Publication Abstract from PubMed The AvrRpt2 protein of the phytopathogenic bacterium Erwinia amylovora (AvrRpt2EA) is a secreted type III effector protein, which is recognised by the FB_MR5 resistance protein of Malusxrobusta 5, the only identified resistance protein from a Malus species preventing E. amylovora infection. The crystal structure of the immature catalytic domain of AvrRpt2EA, a C70 family cysteine protease and type III effector, was determined to a resolution of 1.85A. The structure provides insights into the cyclophilin-dependent activation of AvrRpt2, and identifies a cryptic leucine of a non-canonical cyclophilin binding motif. The structure also suggests that residue Cys156, responsible for the gene induced resistance, is not involved in substrate determination, and hints that recognition by FB_MR5 is due to direct interaction. The structure of Erwinia amylovora AvrRpt2 provides insight into protein maturation and induced resistance to fire blight by Malusxrobusta 5.,Bartho JD, Demitri N, Bellini D, Flachowsky H, Peil A, Walsh MA, Benini S J Struct Biol. 2019 Mar 27. pii: S1047-8477(19)30064-4. doi:, 10.1016/j.jsb.2019.03.010. PMID:30928616 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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