Structural highlights
Publication Abstract from PubMedPseudouridine (Psi) is the most common chemical modification present in RNA. In general, Psi increases the thermodynamic stability of RNA. However, the degree of stabilization depends on the sequence and structural context. To explain experimentally observed sequence dependence of the effect of Psi on the thermodynamic stability of RNA duplexes, we investigated the structure, dynamics and hydration of RNA duplexes with an internal Psi-A base pair in different nearest-neighbor sequence contexts. The structures of two RNA duplexes containing 5'-GPsiC/3'-CAG and 5'-CPsiG/3'-GAC motifs were determined using NMR spectroscopy. To gain insight into the effect of Psi on duplex dynamics and hydration, we performed molecular dynamics (MD) simulations of RNA duplexes with 5'-GPsiC/3'-CAG, 5'-CPsiG/3'-GAC, 5'-APsiU/3'-UAA and 5'-UPsiA/3'-AAU motifs and their unmodified counterparts. Our results showed a subtle impact from Psi modification on the structure and dynamics of the RNA duplexes studied. The MD simulations confirmed the change in hydration pattern when U is replaced with Psi. Quantum chemical calculations showed that the replacement of U with Psi affected the intrinsic stacking energies at the base pair steps depending on the sequence context. The calculated intrinsic stacking energies help to explain the experimentally observed sequence dependent changes in the duplex stability from Psi modification. Computational and NMR studies of RNA duplexes with an internal pseudouridine-adenosine base pair.,Deb I, Popenda L, Sarzynska J, Malgowska M, Lahiri A, Gdaniec Z, Kierzek R Sci Rep. 2019 Nov 7;9(1):16278. doi: 10.1038/s41598-019-52637-0. PMID:31700156[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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