6i50

Structure of Eiger TNF from S. frugiperda

spinqualitylabelsall models 6i50, resolution 1.69Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6i50 is a 1 chain structure with sequence from Spodoptera frugiperda. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.69Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A2H1VTZ0_SPOFR Publication Abstract from PubMed The tumour necrosis factor alpha (TNFalpha) superfamily of proteins are critical in numerous biological processes, such as in development and immunity. Eiger is the sole TNFalpha member described in arthropods such as in the important model organism Drosophila. To date there are no structural data on any Eiger protein. Here we present the structure of the TNF domain of Eiger from the fall armyworm Spodoptera frugiperda (SfEiger) to 1.7 A from a serendipitously obtained crystal without prior knowledge of the protein sequence. Our structure confirms that canonical trimerization is conserved from ancestral TNFs and points towards a mode of receptor engagement. Furthermore, we observe numerous surface histidines on SfEiger, potentially acting as pH switches following internalization into endosomes. Our data contributes to the genome annotation of S. frugiperda, a voracious agricultural pest, and can serve as a basis for future structure-function investigations of the TNF system in related arthropods such as Drosophila. High-resolution crystal structure of arthropod Eiger TNF suggests a mode of receptor engagement and altered surface charge within endosomes.,Bertinelli M, Paesen GC, Grimes JM, Renner M Commun Biol. 2019 Aug 6;2:293. doi: 10.1038/s42003-019-0541-0. eCollection 2019. PMID:31396573 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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