6i60

Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum

spinqualitylabelsall models 6i60, resolution 2.74Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6i60 is a 2 chain structure with sequence from Dictyoglomus thermophilum H-6-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.743Å Ligands: AE3, PGE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B5YC64_DICT6 Publication Abstract from PubMed alpha-l-Rhamnosidases are catalysts of industrial tremendous interest, but their uses are still somewhat limited by their poor thermal stabilities and selectivities. The thermophilic DtRha from Dictyoglomus thermophilum was cloned, and the recombinant protein was easily purified to homogeneity to afford 4.5 mg/L culture of biocatalyst. Michaelis-Menten parameters demonstrated it to be fully specific for alpha-l-rhamnose. Most significantly, DtRha demonstrated to have a stronger preference for alpha(1 --> 2) linkage rather than alpha(1 --> 6) linkage when removing rhamnosyl moiety from natural flavonoids. This selectivity was fully explained by the difference of binding of the corresponding substrates in the active site of the protein. Biochemical Characterization of the alpha-l-Rhamnosidase DtRha from Dictyoglomus thermophilum: Application to the Selective Derhamnosylation of Natural Flavonoids.,Guillotin L, Kim H, Traore Y, Moreau P, Lafite P, Coquoin V, Nuccio S, de Vaumas R, Daniellou R ACS Omega. 2019 Jan 24;4(1):1916-1922. doi: 10.1021/acsomega.8b03186. eCollection, 2019 Jan 31. PMID:31459445 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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