6ibi

Copper binding protein from Laetisaria arvalis (LaX325)

spinqualitylabelsall models 6ibi, resolution 2.08Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6ibi is a 4 chain structure with sequence from Laetisaria arvalis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.08Å Ligands: 1PE, CU, IMD, NAG, PGE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function X325_WAIAR Publication Abstract from PubMed Lytic polysaccharide monooxygenases (LPMOs) are copper-containing enzymes that play a key role in the oxidative degradation of various biopolymers such as cellulose and chitin. While hunting for new LPMOs, we identified a new family of proteins, defined here as X325, in various fungal lineages. The three-dimensional structure of X325 revealed an overall LPMO fold and a His brace with an additional Asp ligand to Cu(II). Although LPMO-type activity of X325 members was initially expected, we demonstrated that X325 members do not perform oxidative cleavage of polysaccharides, establishing that X325s are not LPMOs. Investigations of the biological role of X325 in the ectomycorrhizal fungus Laccaria bicolor revealed exposure of the X325 protein at the interface between fungal hyphae and tree rootlet cells. Our results provide insights into a family of copper-containing proteins, which is widespread in the fungal kingdom and is evolutionarily related to LPMOs, but has diverged to biological functions other than polysaccharide degradation. A fungal family of lytic polysaccharide monooxygenase-like copper proteins.,Labourel A, Frandsen KEH, Zhang F, Brouilly N, Grisel S, Haon M, Ciano L, Ropartz D, Fanuel M, Martin F, Navarro D, Rosso MN, Tandrup T, Bissaro B, Johansen KS, Zerva A, Walton PH, Henrissat B, Leggio LL, Berrin JG Nat Chem Biol. 2020 Jan 13. pii: 10.1038/s41589-019-0438-8. doi:, 10.1038/s41589-019-0438-8. PMID:31932718 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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