6iej

The C2 domain of cytosolic phospholipase A2 alpha bound to phosphatidylcholine

spinqualitylabelsall models 6iej, resolution 2.21Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6iej is a 3 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.206Å Ligands: CA, HXG, MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function PA24A_CHICK Publication Abstract from PubMed Ca(2+)-stimulated translocation of cytosolic phospholipase A2alpha (cPLA2alpha) to the Golgi induces arachidonic acid production, the rate-limiting step in pro-inflammatory eicosanoid synthesis. Structural insights into the cPLA2alpha preference for phosphatidylcholine (PC)-enriched membranes have remained elusive. Here, we report cPLA2alpha C2-domain structure (2.2A resolution) containing bound 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) and Ca(2+) ions. Two Ca(2+) are complexed at locations previously reported for lipid-free C2-domain. One of these Ca(2+) along with a third Ca(2+) bridge the C2-domain to the DHPC phosphate group, which also interacts with Asn65. Tyr96 plays a key role in lipid headgroup recognition via cation-pi interaction with the PC trimethylammonium group. Mutagenesis analyses confirm Tyr96 and Asn65 function in PC binding selectivity by C2-domain and regulation of cPLA2alpha activity. The differing DHPC-binding mode of cPLA2alpha C2-domain, compared to phosphatidylserine or phosphatidylinositol 4,5-bisphosphate binding by other C2-domains, expands and deepens knowledge of lipid-binding mechanisms mediated by C2-domains. Structural basis of phosphatidylcholine recognition by the C2-domain of cytosolic phospholipase A2alpha.,Hirano Y, Gao YG, Stephenson DJ, Vu NT, Malinina L, Simanshu DK, Chalfant CE, Patel DJ, Brown RE Elife. 2019 May 3;8. pii: 44760. doi: 10.7554/eLife.44760. PMID:31050338 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Phospholipase A2 3D structures

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