6ing

A complex structure of H25A mutant of glycosyltransferase with UDP

spinqualitylabelsall models 6ing, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6ing is a 1 chain structure with sequence from Stevia rebaudiana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.698Å Ligands: GOL, UDP Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function U76G1_STERE Publication Abstract from PubMed Diets high in sugar are recognized as a serious health problem, and there is a drive to reduce their consumption. Steviol glycosides are natural zero-calorie sweeteners, but the most desirable ones are biosynthesized with low yields. UGT76G1 catalyzes the beta (1-3) addition of glucose to steviol glycosides, which gives them the preferred taste. UGT76G1 is able to transfer glucose to multiple steviol substrates yet remains highly specific in the glycosidic linkage it creates. Here, we report multiple complex structures of the enzyme combined with biochemical data, which reveal that the enzyme utilizes hydrophobic interactions for substrate recognition. The lack of a strict three-dimensional recognition arrangement, typical of hydrogen bonds, permits two different orientations for beta (1-3) sugar addition. The use of hydrophobic recognition is unusual in a regio- and stereo-specific catalysis. Harnessing such non-specific hydrophobic interactions could have wide applications in the synthesis of complex glycoconjugates. Hydrophobic recognition allows the glycosyltransferase UGT76G1 to catalyze its substrate in two orientations.,Yang T, Zhang J, Ke D, Yang W, Tang M, Jiang J, Cheng G, Li J, Cheng W, Wei Y, Li Q, Naismith JH, Zhu X Nat Commun. 2019 Jul 19;10(1):3214. doi: 10.1038/s41467-019-11154-4. PMID:31324778 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Glycosyltransferase 3D structures

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