6io6

Silver-bound Glyceraldehyde-3-phosphate dehydrogenase A at non-catalytic site

spinqualitylabelsall models 6io6, resolution 2.64Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6io6 is a 1 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.64Å Ligands: AG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function G3P1_ECOLI Publication Abstract from PubMed Silver has long been used as an antibacterial agent, yet its molecular targets remain largely unknown. Using a custom-designed coupling of gel electrophoresis with inductively coupled plasma mass spectrometry (GE-ICP-MS), we identified six silver-binding proteins in E. coli. The majority of the identified proteins are associated with the central carbon metabolism of E. coli. Among them, we unveil that GAPDH, an essential enzyme in glycolysis, serves as a vital target of Ag(+) in E. coli for the first time. We demonstrate that silver inhibits the enzymatic function of GAPDH through targeting Cys149 in its catalytic site. The X-ray structure reveals that Ag(+) coordinates to Cys149 and His176 with a quasi-linear geometry (S-Ag-N angle of 157 degrees ). And unexpectedly, two Ag(+) ions coordinate to Cys288 in the non-catalytic site with weak argentophilic interaction (Ag...Ag distance of 2.9 A). This is the first report on antimicrobial Ag(+) targeting a key enzyme in the glycolytic pathway of E. coli. The findings expand our knowledge on the mode of action and bio-coordination chemistry of silver, particularly silver-targeting residues in proteins at the atomic level. Antimicrobial silver targets glyceraldehyde-3-phosphate dehydrogenase in glycolysis of E. coli.,Wang H, Wang M, Yang X, Xu X, Hao Q, Yan A, Hu M, Lobinski R, Li H, Sun H Chem Sci. 2019 Jun 24;10(30):7193-7199. doi: 10.1039/c9sc02032b. eCollection 2019, Aug 14. PMID:31588287 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Glyceraldehyde-3-phosphate dehydrogenase 3D structures

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