6iya

Structure of the DNA binding domain of antitoxin CopASO

spinqualitylabelsall models 6iya, resolution 3.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6iya is a 6 chain structure with sequence from Shewanella oneidensis MR-1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q8EGZ2_SHEON Publication Abstract from PubMed Toxin-antitoxin (TA) systems play critical roles in the environment adaptation of bacteria. Allosteric coupling between the N-terminal DNA-binding domain and the C-terminal toxin-binding domain of antitoxins contributes to conditional cooperativity in the functioning of type II TA. Herein, using circular dichroism (CD), nuclear magnetic resonance (NMR), X-ray crystallography, and size exclusion chromatography (SEC), the structure and DNA binding of CopASO, a newly identified type II antitoxin in Shewanella oneidensis, were investigated. Our data show that CopASO is a typical RHH antitoxin with an ordered N-terminal domain and a disordered C-terminal domain, and furthermore indicate that the C-terminal domain facilitates DNA binding of the N-terminal domain, which in turn induces the C-terminal domain to fold and associate. Structure and allosteric coupling of type antitoxin CopASO.,Zhao R, Li Q, Zhang J, Li F, Yao J, Zhang J, Liu L, Wang X, Zhang X Biochem Biophys Res Commun. 2019 Jul 5;514(4):1122-1127. doi:, 10.1016/j.bbrc.2019.05.049. Epub 2019 May 14. PMID:31101334 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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