Structural highlights
Publication Abstract from PubMedOBJECTIVES: In order to find antimicrobial resistance gene(s) pre-dating the use of antibiotics through metagenomics, functional screening of a metagenomic library from the deep-seep sediments of Edison Seamount (ca. 10000 years old) was performed. METHODS: Among 60 antimicrobial-resistant clones, a single clone with the highest minimum inhibitory concentration (MIC) for ampicillin was selected. Sequence analysis revealed a new metallo-beta-lactamase (MBL) gene, designated as blaPNGM-1. PNGM-1 retains a zinc ion-binding motif (H116XH118XD120H121, H196 and H263), conserved in subclass B3 MBLs. The catalytic parameters of purified PNGM-1 and the MICs of beta-lactams for Escherichia coli TOP10 transformants harbouring the blaPNGM-1 gene were assessed. RESULTS: Antimicrobial susceptibility testing indicated reduced susceptibility to penicillins, narrow- and extended-spectrum cephalosporins, and carbapenems in E. coli TOP10 transformants harbouring the blaPNGM-1 gene. In addition, kinetic analyses revealed that PNGM-1 hydrolysed almost all beta-lactams. CONCLUSIONS: The PNGM-1 enzyme is the first case of a subclass B3 MBL derived from a functional metagenomic library of a deep-sea sediment that pre-dates the antibiotic era. PNGM-1, a novel subclass B3 metallo-beta-lactamase from a deep-sea sediment metagenome.,Park KS, Kim TY, Kim JH, Lee JH, Jeon JH, Karim AM, Malik SK, Lee SH J Glob Antimicrob Resist. 2018 Sep;14:302-305. doi: 10.1016/j.jgar.2018.05.021., Epub 2018 May 26. PMID:29842976[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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